Gene disruption with PCR products in Saccharomyces cerevisiae.

Journal Article (Journal Article)

We describe here the generation of gene disruption constructs using PCR amplification of selectable markers with primers that provide homology to the target gene of interest. We find that regions of homology as short as 38 to 50 bp suffice to mediate homologous recombination in yeast. We describe applications of this technology to three specific yeast genes that would have been difficult to disrupt with current methods. By dispensing with the need to either clone the gene of interest or engineer a standard disruption construct, this method should facilitate analysis of sequenced genes of unknown function, which will soon include the entire yeast genome.

Full Text

Duke Authors

Cited Authors

  • Lorenz, MC; Muir, RS; Lim, E; McElver, J; Weber, SC; Heitman, J

Published Date

  • May 26, 1995

Published In

Volume / Issue

  • 158 / 1

Start / End Page

  • 113 - 117

PubMed ID

  • 7789793

International Standard Serial Number (ISSN)

  • 0378-1119

Digital Object Identifier (DOI)

  • 10.1016/0378-1119(95)00144-u


  • eng

Conference Location

  • Netherlands