Protection from nitrosative stress by yeast flavohemoglobin.
Published
Journal Article
Yeast hemoglobin was discovered close to half a century ago, but its function has remained unknown. Herein, we report that this flavohemoglobin protects Saccharomyces cerevisiae from nitrosative stress. Deletion of the flavohemoglobin gene (YHB1) abolished the nitric oxide (NO)-consuming activity of yeast cells. Levels of protein nitrosylation were more than 10-fold higher in yhb1 mutant yeast than in isogenic wild-type cells after incubation with NO donors. Growth of mutant cells was inhibited by a nitrosative challenge that had little effect on wild-type cells, whereas the resistance of mutant cells to oxidative stress was unimpaired. Protection conferred by yeast flavohemoglobin against NO and S-nitrosothiols was seen under both anaerobic and aerobic conditions, consistent with a primary function in NO detoxification. A phylogenetic analysis indicated that protection from nitrosative stress is likely to be a conserved function among microorganismal flavohemoglobins. Flavohemoglobin is therefore a potential target for antimicrobial therapy.
Full Text
Duke Authors
Cited Authors
- Liu, L; Zeng, M; Hausladen, A; Heitman, J; Stamler, JS
Published Date
- April 25, 2000
Published In
Volume / Issue
- 97 / 9
Start / End Page
- 4672 - 4676
PubMed ID
- 10758168
Pubmed Central ID
- 10758168
International Standard Serial Number (ISSN)
- 0027-8424
Digital Object Identifier (DOI)
- 10.1073/pnas.090083597
Language
- eng
Conference Location
- United States