A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 protein.

Journal Article (Journal Article)

A mammalian protein of approximately 220 kDa (p220) was UV-crosslinked to precursor mRNAs (pre-mRNAs) under splicing conditions. The kinetics and biochemical requirements of the UV-crosslinking of p220 corresponded to the kinetics and biochemical requirements of spliceosome formation. On Western blots, antibodies against the yeast splicing factor PRP8 recognized a doublet of proteins, the faster migrating of which comigrated with p220. Furthermore, UV-crosslinked p220 was immunoprecipitated with anti-PRP8 antisera. These results suggest structural conservation of the splicing factor PRP8 from yeast to mammals and show that this protein is in close proximity to the pre-mRNA in the spliceosome.

Full Text

Duke Authors

Cited Authors

  • Garcia-Blanco, MA; Anderson, GJ; Beggs, J; Sharp, PA

Published Date

  • April 1990

Published In

Volume / Issue

  • 87 / 8

Start / End Page

  • 3082 - 3086

PubMed ID

  • 2139226

Pubmed Central ID

  • PMC53838

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.87.8.3082


  • eng

Conference Location

  • United States