Transcriptional cofactor CA150 regulates RNA polymerase II elongation in a TATA-box-dependent manner.

Journal Article

Tat protein strongly activates transcription from the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) by enhancing the elongation efficiency of RNA polymerase II complexes. Tat-mediated transcriptional activation requires cellular cofactors and specific cis-acting elements within the HIV-1 promoter, among them a functional TATA box. Here, we have investigated the mechanism by which one of these cofactors, termed CA150, regulates HIV-1 transcription in vivo. We present a series of functional assays that demonstrate that the regulation of the HIV-1 LTR by CA150 has the same functional requirements as the activation by Tat. We found that CA150 affects elongation of transcription complexes assembled on the HIV-1 promoter in a TATA-box-dependent manner. We discuss the data in terms of the involvement of CA150 in the regulation of Tat-activated HIV-1 gene expression. In addition, we also provide evidence suggesting a role for CA150 in the regulation of cellular transcriptional processes.

Full Text

Duke Authors

Cited Authors

  • Suñé, C; Garcia-Blanco, MA

Published Date

  • July 1999

Published In

Volume / Issue

  • 19 / 7

Start / End Page

  • 4719 - 4728

PubMed ID

  • 10373521

International Standard Serial Number (ISSN)

  • 0270-7306

Language

  • eng

Conference Location

  • United States