Transcriptional cofactor CA150 regulates RNA polymerase II elongation in a TATA-box-dependent manner.
Tat protein strongly activates transcription from the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) by enhancing the elongation efficiency of RNA polymerase II complexes. Tat-mediated transcriptional activation requires cellular cofactors and specific cis-acting elements within the HIV-1 promoter, among them a functional TATA box. Here, we have investigated the mechanism by which one of these cofactors, termed CA150, regulates HIV-1 transcription in vivo. We present a series of functional assays that demonstrate that the regulation of the HIV-1 LTR by CA150 has the same functional requirements as the activation by Tat. We found that CA150 affects elongation of transcription complexes assembled on the HIV-1 promoter in a TATA-box-dependent manner. We discuss the data in terms of the involvement of CA150 in the regulation of Tat-activated HIV-1 gene expression. In addition, we also provide evidence suggesting a role for CA150 in the regulation of cellular transcriptional processes.
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Related Subject Headings
- tat Gene Products, Human Immunodeficiency Virus
- Transcriptional Elongation Factors
- Transcription, Genetic
- Transcription Factors
- Trans-Activators
- TATA-Box Binding Protein
- TATA Box
- RNA Polymerase II
- Promoter Regions, Genetic
- Humans
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- tat Gene Products, Human Immunodeficiency Virus
- Transcriptional Elongation Factors
- Transcription, Genetic
- Transcription Factors
- Trans-Activators
- TATA-Box Binding Protein
- TATA Box
- RNA Polymerase II
- Promoter Regions, Genetic
- Humans