Regulation of 2',5'-oligoadenylate synthetase gene expression by interferons and platelet-derived growth factor.

Journal Article (Journal Article)

In murine BALB/c 3T3 cell cultures, either beta interferon or platelet-derived growth factor (PDGF) enhanced expression of the 2',5'-oligoadenylate synthetase mRNA and protein. The time course of induction in response to beta interferon was similar to that in response to PDGF. Of several growth factors known to be present in clotted blood serum (i.e., epidermal growth factor, transforming growth factor beta, and PDGF), only PDGF enhanced expression of 2',5'-oligoadenylate synthetase. The linkage of an interferon response element-containing segment from the 5'-flanking region of a human or murine 2',-5'-oligoadenylate synthetase gene made a heterologous gene responsive to interferon. The expression of such a gene construct in transfected cells was also induced by PDGF. Induction by PDGF was inhibited by mono- or polyclonal antibodies to murine interferon, which suggested that induction by PDGF requires interferon. Both PDGF and interferon induced nuclear factors that bound to this interferon response element-containing segment in vitro.

Full Text

Duke Authors

Cited Authors

  • Garcia-Blanco, MA; Lengyel, P; Morrison, E; Brownlee, C; Stiles, CD; Rutherford, M; Hannigan, G; Williams, BR

Published Date

  • March 1989

Published In

Volume / Issue

  • 9 / 3

Start / End Page

  • 1060 - 1068

PubMed ID

  • 2542764

Pubmed Central ID

  • PMC362696

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.9.3.1060-1068.1989


  • eng

Conference Location

  • United States