Protein-protein interactions and 5'-splice-site recognition in mammalian mRNA precursors.

Journal Article (Journal Article)

Exactly how specific splice sites are recognized during the processing of complex precursor messenger RNAs is not clear. Small nuclear ribonucleoprotein particles (snRNPs) are involved, but are not sufficient by themselves to define splice sites. Now a human protein essential for splicing in vitro, called alternative splicing factor/splicing factor 2, is shown to cooperate with the U1 snRNP particle in binding pre-mRNA. This cooperation is probably achieved by specific interactions between the arginine/serine-rich domain of the splicing factor and a similar region in a U1 snRNP-specific protein.

Full Text

Duke Authors

Cited Authors

  • Kohtz, JD; Jamison, SF; Will, CL; Zuo, P; Lührmann, R; Garcia-Blanco, MA; Manley, JL

Published Date

  • March 10, 1994

Published In

Volume / Issue

  • 368 / 6467

Start / End Page

  • 119 - 124

PubMed ID

  • 8139654

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/368119a0


  • eng

Conference Location

  • England