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Transcriptional trans activation by human immunodeficiency virus type 1 Tat requires specific coactivators that are not basal factors.

Publication ,  Journal Article
Suñé, C; García-Blanco, MA
Published in: Journal of virology
May 1995

Expression of human immunodeficiency virus type 1 (HIV-1) genes is regulated by the trans activator Tat. Tat exerts its effects by increasing the rate of transcription, but the mechanism by which it does so is still unknown. To study the cellular factors required for Tat trans activation, we have expressed functional Gst-Tat fusion protein and used it to construct affinity columns. Our findings are as follows. (i) A Gst-Tat affinity matrix depleted HeLa nuclear extracts of a factor(s) required for Tat function. A Tat mutant bearing the missense mutation lysine to alanine at position 41 was incapable of this depletion. (ii) Tat trans activation was recovered by addition of unfractionated nuclear extract, the 0.5 M KCl elution fraction from the Tat affinity column, or sedimentation gradient fractions of HeLa extracts. The activity from the gradients sedimented with an apparent molecular mass of 200 kDa. (iii) Tat trans activation could not be recovered by use of recombinant human TATA-binding protein or partially purified TFIID. (iv) trans activation by Tat was blocked by heating of the nuclear extract under conditions in which basal transcription was not decreased. Our data demonstrate for the first time the existence of unique Tat coactivators distinct from factors required for general basal transcription.

Published In

Journal of virology

DOI

EISSN

1098-5514

ISSN

0022-538X

Publication Date

May 1995

Volume

69

Issue

5

Start / End Page

3098 / 3107

Related Subject Headings

  • tat Gene Products, Human Immunodeficiency Virus
  • Virology
  • Transcriptional Activation
  • Transcription Factors
  • Transcription Factor TFIID
  • Temperature
  • TATA-Box Binding Protein
  • Recombinant Fusion Proteins
  • Promoter Regions, Genetic
  • Humans
 

Citation

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Suñé, C., & García-Blanco, M. A. (1995). Transcriptional trans activation by human immunodeficiency virus type 1 Tat requires specific coactivators that are not basal factors. Journal of Virology, 69(5), 3098–3107. https://doi.org/10.1128/jvi.69.5.3098-3107.1995
Suñé, C., and M. A. García-Blanco. “Transcriptional trans activation by human immunodeficiency virus type 1 Tat requires specific coactivators that are not basal factors.Journal of Virology 69, no. 5 (May 1995): 3098–3107. https://doi.org/10.1128/jvi.69.5.3098-3107.1995.
Suñé, C., and M. A. García-Blanco. “Transcriptional trans activation by human immunodeficiency virus type 1 Tat requires specific coactivators that are not basal factors.Journal of Virology, vol. 69, no. 5, May 1995, pp. 3098–107. Epmc, doi:10.1128/jvi.69.5.3098-3107.1995.

Published In

Journal of virology

DOI

EISSN

1098-5514

ISSN

0022-538X

Publication Date

May 1995

Volume

69

Issue

5

Start / End Page

3098 / 3107

Related Subject Headings

  • tat Gene Products, Human Immunodeficiency Virus
  • Virology
  • Transcriptional Activation
  • Transcription Factors
  • Transcription Factor TFIID
  • Temperature
  • TATA-Box Binding Protein
  • Recombinant Fusion Proteins
  • Promoter Regions, Genetic
  • Humans