Imaging alternative splicing in living cells.


Journal Article

We have developed an in vivo reporter of alternative splicing decisions that allows for the determination of FGF-R2 splicing patterns without the destruction of cells. This method has broad applications, including the study of other alternatively spliced genes in tissue culture and in whole animals, and may be useful in creating imaging markers for the study of tumor progression and metastasis. In this chapter, the authors present one example of this method using fluorescence reporters. As with any new assay, a series of experiments were performed to validate the method. This chapter documents some of these experiments.

Full Text

Duke Authors

Cited Authors

  • Wagner, EJ; Baines, A; Albrecht, T; Brazas, RM; Garcia-Blanco, MA

Published Date

  • 2004

Published In

Volume / Issue

  • 257 /

Start / End Page

  • 29 - 46

PubMed ID

  • 14769994

Pubmed Central ID

  • 14769994

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1385/1-59259-750-5:029


  • eng

Conference Location

  • United States