Analysis of the individual regulatory components of the IncFII plasmid replication control system.

Journal Article (Journal Article)

Replication of the IncFII plasmid NR1 is controlled by regulating the amount of synthesis of the repA1 initiator protein at both the transcriptional and translational levels. We have examined mutations which have altered each of these levels of regulation, resulting in different plasmid copy numbers. The genes which encode each of the individual wild-type or mutant regulatory components from the replication control region of NR1 have been cloned independently into pBR322 vectors, and their effects in trans, either individually or in various combinations, on plasmid incompatibility, stability, copy number, and repA1 gene expression have been defined.

Full Text

Duke Authors

Dong, Xinnian

Cited Authors

Womble, DD; Dong, X; Luckow, VA; Wu, RP; Rownd, RH

Published Date

February 1985

Published In

Journal of Bacteriology

Volume / Issue

161 / 2

Start / End Page

534 - 543

PubMed ID

3155721

Pubmed Central ID

PMC214915

Electronic International Standard Serial Number (EISSN)

1098-5530

International Standard Serial Number (ISSN)

0021-9193

Digital Object Identifier (DOI)

10.1128/jb.161.2.534-543.1985

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