Revision of standards for adjusting the cation content of Mueller-Hinton broth for testing susceptibility of Pseudomonas aeruginosa to aminoglycosides.

Journal Article (Journal Article)

A multilaboratory study was undertaken to reassess the amount of calcium and magnesium that should be added to Mueller-Hinton broth when testing Pseudomonas aeruginosa against amikacin, gentamicin, isepamicin, netilmicin, and tobramycin. To achieve parity with agar dilution tests, cation-adjusted broth should contain 20 to 25 mg of calcium and 10 to 12.5 mg of magnesium per liter rather than the 50- and 25-mg/liter supplements recommended previously. For quality control of tests with contemporary media, MIC control limits should be adjusted by lowering the current MIC limits by at least 1 doubling-dilution interval.

Full Text

Duke Authors

Cited Authors

  • Barry, AL; Reller, LB; Miller, GH; Washington, JA; Schoenknect, FD; Peterson, LR; Hare, RS; Knapp, C

Published Date

  • March 1992

Published In

Volume / Issue

  • 30 / 3

Start / End Page

  • 585 - 589

PubMed ID

  • 1551973

Pubmed Central ID

  • PMC265114

International Standard Serial Number (ISSN)

  • 0095-1137

Digital Object Identifier (DOI)

  • 10.1128/jcm.30.3.585-589.1992


  • eng

Conference Location

  • United States