Effect of incubation atmosphere and temperature on isolation of Campylobacter jejuni from human stools.

Journal Article (Journal Article)

To determine the optimal conditions for isolation of Campylobacter jejuni from human fecal specimens, we compared incubation atmospheres that contained about 5, 10, and 15% oxygen with the 17% oxygen produced in candle jars and also compared incubation temperatures of 37 and 42 degrees C. At 42 degrees C, C. jejuni was isolated from all 16 specimens; however, colony sizes were larger when plates were incubated in 5 and 10% oxygen than in the other two atmospheres. At 37 degrees C some positive cultures were missed in 15% oxygen and in the candle jar. The largest colony sizes were obtained in 5% oxygen. For each atmospheric condition tested, the colonies were larger at 42 than at 37 degrees C. When incubation is done at 42 degrees C, use of a candle jar is adequate; however, at 37 degrees C candle jars should not be used for isolation of C. jejuni from human feces.

Full Text

Duke Authors

Cited Authors

  • Wang, WL; Luechtefeld, NW; Blaser, MJ; Reller, LB

Published Date

  • April 1, 1983

Published In

Volume / Issue

  • 29 / 4

Start / End Page

  • 468 - 470

PubMed ID

  • 6850426

International Standard Serial Number (ISSN)

  • 0008-4166

Digital Object Identifier (DOI)

  • 10.1139/m83-075


  • eng

Conference Location

  • Canada