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Effect of incubation atmosphere and temperature on isolation of Campylobacter jejuni from human stools.

Publication ,  Journal Article
Wang, WL; Luechtefeld, NW; Blaser, MJ; Reller, LB
Published in: Can J Microbiol
April 1983

To determine the optimal conditions for isolation of Campylobacter jejuni from human fecal specimens, we compared incubation atmospheres that contained about 5, 10, and 15% oxygen with the 17% oxygen produced in candle jars and also compared incubation temperatures of 37 and 42 degrees C. At 42 degrees C, C. jejuni was isolated from all 16 specimens; however, colony sizes were larger when plates were incubated in 5 and 10% oxygen than in the other two atmospheres. At 37 degrees C some positive cultures were missed in 15% oxygen and in the candle jar. The largest colony sizes were obtained in 5% oxygen. For each atmospheric condition tested, the colonies were larger at 42 than at 37 degrees C. When incubation is done at 42 degrees C, use of a candle jar is adequate; however, at 37 degrees C candle jars should not be used for isolation of C. jejuni from human feces.

Duke Scholars

Published In

Can J Microbiol

DOI

ISSN

0008-4166

Publication Date

April 1983

Volume

29

Issue

4

Start / End Page

468 / 470

Location

Canada

Related Subject Headings

  • Temperature
  • Oxygen
  • Microbiology
  • Humans
  • Feces
  • Campylobacter fetus
  • Campylobacter
  • 3207 Medical microbiology
  • 3107 Microbiology
  • 1108 Medical Microbiology
 

Citation

APA
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ICMJE
MLA
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Wang, W. L., Luechtefeld, N. W., Blaser, M. J., & Reller, L. B. (1983). Effect of incubation atmosphere and temperature on isolation of Campylobacter jejuni from human stools. Can J Microbiol, 29(4), 468–470. https://doi.org/10.1139/m83-075
Wang, W. L., N. W. Luechtefeld, M. J. Blaser, and L. B. Reller. “Effect of incubation atmosphere and temperature on isolation of Campylobacter jejuni from human stools.Can J Microbiol 29, no. 4 (April 1983): 468–70. https://doi.org/10.1139/m83-075.
Wang WL, Luechtefeld NW, Blaser MJ, Reller LB. Effect of incubation atmosphere and temperature on isolation of Campylobacter jejuni from human stools. Can J Microbiol. 1983 Apr;29(4):468–70.
Wang, W. L., et al. “Effect of incubation atmosphere and temperature on isolation of Campylobacter jejuni from human stools.Can J Microbiol, vol. 29, no. 4, Apr. 1983, pp. 468–70. Pubmed, doi:10.1139/m83-075.
Wang WL, Luechtefeld NW, Blaser MJ, Reller LB. Effect of incubation atmosphere and temperature on isolation of Campylobacter jejuni from human stools. Can J Microbiol. 1983 Apr;29(4):468–470.

Published In

Can J Microbiol

DOI

ISSN

0008-4166

Publication Date

April 1983

Volume

29

Issue

4

Start / End Page

468 / 470

Location

Canada

Related Subject Headings

  • Temperature
  • Oxygen
  • Microbiology
  • Humans
  • Feces
  • Campylobacter fetus
  • Campylobacter
  • 3207 Medical microbiology
  • 3107 Microbiology
  • 1108 Medical Microbiology