Development of a standardized screening method for detection of vancomycin-resistant enterococci.

Published

Journal Article

The incidence of vancomycin resistance among enterococci is increasing in the United States and elsewhere in the world, but automated susceptibility testing methods have difficulty detecting resistance expressed by certain strains. The agar screening method described by Willey et al. (B. M. Willey, B. N. Kreiswirth, A. E. Simor, G. Williams, S. R. Scriver, A. Phillips, and D. E. Low, J. Clin. Microbiol. 30:1621-1624, 1992) has been proposed as a reliable method for confirming vancomycin resistance. In this study, we investigated various parameters associated with the agar screening method and, on the basis of the findings, established optimum testing conditions for the method. First, to evaluate media and vancomycin concentrations, one laboratory used Mueller-Hinton and brain heart infusion agars supplemented with 4, 6, and 8 micrograms of vancomycin per ml to test 100 genetically characterized enterococcal strains. On the basis of the results obtained, brain heart infusion agar supplemented with 6 micrograms of vancomycin per ml was selected for further study. Subsequently, eight laboratories used the medium to test both reference and clinical isolates. There was very good performance with the reference strains and, among 158 clinical isolates tested, the method demonstrated sensitivity and specificity of 100% and from 96 to 99%, respectively.

Full Text

Duke Authors

Cited Authors

  • Swenson, JM; Clark, NC; Ferraro, MJ; Sahm, DF; Doern, G; Pfaller, MA; Reller, LB; Weinstein, MP; Zabransky, RJ; Tenover, FC

Published Date

  • July 1994

Published In

Volume / Issue

  • 32 / 7

Start / End Page

  • 1700 - 1704

PubMed ID

  • 7929760

Pubmed Central ID

  • 7929760

Electronic International Standard Serial Number (EISSN)

  • 1098-660X

International Standard Serial Number (ISSN)

  • 0095-1137

Language

  • eng