Skip to main content

Controlled evaluation of 5 versus 10 milliliters of blood cultured in aerobic BacT/Alert blood culture bottles.

Publication ,  Journal Article
Weinstein, MP; Mirrett, S; Wilson, ML; Reimer, LG; Reller, LB
Published in: J Clin Microbiol
September 1994

Bottles developed for use in the BacT/Alert automated blood culture system (Organon Teknika Corp., Durham, N.C.) can accept up to 10 ml of blood without falling below a 1:5 ratio of blood to broth. We compared the yield and speed of detection of microorganisms in 13,128 adequately filled, paired, aerobic bottles inoculated with 5 versus 10 ml of blood at three university hospitals. A total of 798 microorganisms causing sepsis grew in one or both bottles. The overall recovery of microorganisms from 10-ml samples exceeded that from 5-ml samples (P < 0.001); the increased yield attributed to the additional 5 ml in the samples was 7.2%. The increased yield from 10-ml inocula was most marked for Escherichia coli (P < 0.01) and other members of the family Enterobacteriaceae (P < 0.001). Ten-milliliter samples did not yield more gram-positive bacteria, nonfermentative gram-negative rods, or yeasts. When both bottles were positive, the bottles inoculated with 10 ml of blood showed growth sooner (P < 0.001). Earlier detection with 10-ml inocula was especially notable for coagulase-negative staphylococci (P < 0.001), streptococci (P < 0.001), E. coli (P < 0.025), and other members of the family Enterobacteriaceae (P < 0.025). We conclude that an increase in the volume of blood inoculated into BacT/Alert aerobic blood culture bottles from 5 to 10 ml will increase the overall yield and the speed of detection of clinically important blood pathogens.

Duke Scholars

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

September 1994

Volume

32

Issue

9

Start / End Page

2103 / 2106

Location

United States

Related Subject Headings

  • Staphylococcus aureus
  • Mycology
  • Microbiology
  • Humans
  • Fungi
  • Fungemia
  • Evaluation Studies as Topic
  • Escherichia coli
  • Enterobacteriaceae
  • Blood
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Weinstein, M. P., Mirrett, S., Wilson, M. L., Reimer, L. G., & Reller, L. B. (1994). Controlled evaluation of 5 versus 10 milliliters of blood cultured in aerobic BacT/Alert blood culture bottles. J Clin Microbiol, 32(9), 2103–2106. https://doi.org/10.1128/jcm.32.9.2103-2106.1994
Weinstein, M. P., S. Mirrett, M. L. Wilson, L. G. Reimer, and L. B. Reller. “Controlled evaluation of 5 versus 10 milliliters of blood cultured in aerobic BacT/Alert blood culture bottles.J Clin Microbiol 32, no. 9 (September 1994): 2103–6. https://doi.org/10.1128/jcm.32.9.2103-2106.1994.
Weinstein MP, Mirrett S, Wilson ML, Reimer LG, Reller LB. Controlled evaluation of 5 versus 10 milliliters of blood cultured in aerobic BacT/Alert blood culture bottles. J Clin Microbiol. 1994 Sep;32(9):2103–6.
Weinstein, M. P., et al. “Controlled evaluation of 5 versus 10 milliliters of blood cultured in aerobic BacT/Alert blood culture bottles.J Clin Microbiol, vol. 32, no. 9, Sept. 1994, pp. 2103–06. Pubmed, doi:10.1128/jcm.32.9.2103-2106.1994.
Weinstein MP, Mirrett S, Wilson ML, Reimer LG, Reller LB. Controlled evaluation of 5 versus 10 milliliters of blood cultured in aerobic BacT/Alert blood culture bottles. J Clin Microbiol. 1994 Sep;32(9):2103–2106.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

September 1994

Volume

32

Issue

9

Start / End Page

2103 / 2106

Location

United States

Related Subject Headings

  • Staphylococcus aureus
  • Mycology
  • Microbiology
  • Humans
  • Fungi
  • Fungemia
  • Evaluation Studies as Topic
  • Escherichia coli
  • Enterobacteriaceae
  • Blood