Controlled evaluation of the agar-slide and radiometric blood culture systems for the detection of bacteremia and fungemia.

Journal Article (Journal Article)

A commercially available agar-slide blood culture bottle (Septi-Chek; Roche Diagnostics, Div. Hoffman-La Roche, Inc., Nutley, N.J.) was compared with the radiometric blood culture system (BACTEC; Johnston Laboratories, Inc., Towson, Md.) in 8,544 paired blood cultures from adult patients. The systems were inoculated with equal volumes (10 ml) of blood. Overall, there was no statistically significant difference between the two systems in the recovery of clinically important microorganisms, but significantly more members of the family Enterobacteriaceae other than Escherichia coli were detected by the agar-slide system (P less than 0.005). The agar-slide system detected more fungi, and the BACTEC detected more anaerobic bacteria; however, small numbers of recovered organisms precluded statistical significance. When microorganisms grew in both systems, their presence was detected one or more days earlier in the BACTEC (P less than 0.001). More contaminants grew in the agar-slide system (P less than 0.001). Both systems performed well, and either system should provide high yield and prompt detection of positive blood cultures in patients with bacteremia and fungemia if used in an optimal way as recommended by the respective manufacturers.

Full Text

Duke Authors

Cited Authors

  • Weinstein, MP; Reller, LB; Mirrett, S; Stratton, CW; Reimer, LG; Wang, WL

Published Date

  • February 1, 1986

Published In

Volume / Issue

  • 23 / 2

Start / End Page

  • 221 - 225

PubMed ID

  • 3517047

Pubmed Central ID

  • PMC268616

International Standard Serial Number (ISSN)

  • 0095-1137

Digital Object Identifier (DOI)

  • 10.1128/jcm.23.2.221-225.1986


  • eng

Conference Location

  • United States