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Bile-esculin test for presumptive identification of enterococci and streptococci: effects of bile concentration, inoculation technique, and incubation time.

Publication ,  Journal Article
Chuard, C; Reller, LB
Published in: J Clin Microbiol
April 1998

The bile-esculin test is used to differentiate enterococci and group D streptococci from non-group D viridans group streptococci. The effects on test performance of the concentration of bile salts, inoculum, and duration of incubation were examined with 110 strains of enterococci, 30 strains of Streptococcus bovis, and 110 strains of non-group D viridans group streptococci. Optimal sensitivity (> 99%) and specificity (97%) of the bile-esculin test can be obtained with a bile concentration of 40%, a standardized inoculum of 10(6) CFU, and incubation for 24 h.

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Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

April 1998

Volume

36

Issue

4

Start / End Page

1135 / 1136

Location

United States

Related Subject Headings

  • Time Factors
  • Streptococcus
  • Sensitivity and Specificity
  • Microbiology
  • Esculin
  • Enterococcus
  • Bile
  • 3207 Medical microbiology
  • 3202 Clinical sciences
  • 11 Medical and Health Sciences
 

Citation

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Chuard, C., & Reller, L. B. (1998). Bile-esculin test for presumptive identification of enterococci and streptococci: effects of bile concentration, inoculation technique, and incubation time. J Clin Microbiol, 36(4), 1135–1136. https://doi.org/10.1128/JCM.36.4.1135-1136.1998
Chuard, C., and L. B. Reller. “Bile-esculin test for presumptive identification of enterococci and streptococci: effects of bile concentration, inoculation technique, and incubation time.J Clin Microbiol 36, no. 4 (April 1998): 1135–36. https://doi.org/10.1128/JCM.36.4.1135-1136.1998.
Chuard, C., and L. B. Reller. “Bile-esculin test for presumptive identification of enterococci and streptococci: effects of bile concentration, inoculation technique, and incubation time.J Clin Microbiol, vol. 36, no. 4, Apr. 1998, pp. 1135–36. Pubmed, doi:10.1128/JCM.36.4.1135-1136.1998.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

April 1998

Volume

36

Issue

4

Start / End Page

1135 / 1136

Location

United States

Related Subject Headings

  • Time Factors
  • Streptococcus
  • Sensitivity and Specificity
  • Microbiology
  • Esculin
  • Enterococcus
  • Bile
  • 3207 Medical microbiology
  • 3202 Clinical sciences
  • 11 Medical and Health Sciences