Bile-esculin test for presumptive identification of enterococci and streptococci: effects of bile concentration, inoculation technique, and incubation time.

Journal Article (Journal Article)

The bile-esculin test is used to differentiate enterococci and group D streptococci from non-group D viridans group streptococci. The effects on test performance of the concentration of bile salts, inoculum, and duration of incubation were examined with 110 strains of enterococci, 30 strains of Streptococcus bovis, and 110 strains of non-group D viridans group streptococci. Optimal sensitivity (> 99%) and specificity (97%) of the bile-esculin test can be obtained with a bile concentration of 40%, a standardized inoculum of 10(6) CFU, and incubation for 24 h.

Full Text

Duke Authors

Cited Authors

  • Chuard, C; Reller, LB

Published Date

  • April 1998

Published In

Volume / Issue

  • 36 / 4

Start / End Page

  • 1135 - 1136

PubMed ID

  • 9542954

Pubmed Central ID

  • PMC104706

International Standard Serial Number (ISSN)

  • 0095-1137

Digital Object Identifier (DOI)

  • 10.1128/JCM.36.4.1135-1136.1998


  • eng

Conference Location

  • United States