Comparison of CampyPak II with standard 5% oxygen and candle jars for growth of Campylobacter jejuni from human feces.

Published

Journal Article

To determine optimal temperature and atmospheric conditions for isolating Campylobacter jejuni from fecal specimens of humans, we studied six laboratory isolates and 19 fecal specimens that were known to contain C. jejuni. We compared incubations in 5% oxygen, the CampyPak II (BBL Microbiology Systems, Cockeysville, Md.) with 6 plates per jar (CP-6) and 12 plates per jar (CP-12), and candle jars at 37 and 42 degrees C. At both temperatures, the colony sizes for the laboratory strains were larger in the 5% O2 and the CP-6 than under the other two conditions. For the primary isolations, CP-12 failed to detect one and two campylobacters at 42 and 37 degrees C, respectively, whereas the candle jar failed to detect one at 42 degrees C and four at 37 degrees C. Colony size was again larger in the 5% O2 and the CP-6. For all four atmospheric conditions tested, colonies were significantly larger at 42 degrees C than at 37 degrees C. These studies showed that incubation at 42 degrees C in either 5% O2 or the CampyPak II with six plates per jar was optimal for primary isolation of C. jejuni from fecal specimens of humans. The candle jars incubated at 42 degrees C appeared to be satisfactory for primary isolation of C. jejuni from human feces, but incubation at 37 degrees C was not acceptable.

Full Text

Duke Authors

Cited Authors

  • Wang, WL; Luechtefeld, NW; Blaser, MJ; Reller, LB

Published Date

  • August 1, 1982

Published In

Volume / Issue

  • 16 / 2

Start / End Page

  • 291 - 294

PubMed ID

  • 6749892

Pubmed Central ID

  • 6749892

Electronic International Standard Serial Number (EISSN)

  • 1098-660X

International Standard Serial Number (ISSN)

  • 0095-1137

Language

  • eng