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The effects of clinically relevant hyperthermic temperatures on the kinetic binding parameters of a monoclonal antibody.

Publication ,  Journal Article
Hauck, ML; Dewhirst, MW; Zalutsky, MR
Published in: Nucl Med Biol
May 1996

Hyperthermia is a therapeutic modality under investigation for its ability to increase absolute levels of tumor uptake of radiolabeled monoclonal antibodies (MAbs). We have investigated whether hyperthermia may affect the binding parameters of MAbs. The effects of clinically relevant levels of hyperthermia on the kinetic binding parameters were investigated for 81C6, an antibody undergoing Phase I/II clinical trials for the treatment of brain tumors and neoplastic meningitis. No obvious effects of temperature in either the association or dissociation rate constants, nor in the equilibrium constants, were apparent between 37 degrees and 45 degrees C. The improved binding stability of the bivalent form of the MAb was apparent when compared with its monovalent Fab fragment.

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Published In

Nucl Med Biol

DOI

ISSN

0969-8051

Publication Date

May 1996

Volume

23

Issue

4

Start / End Page

551 / 557

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Osteosarcoma
  • Nuclear Medicine & Medical Imaging
  • Mice, Nude
  • Mice
  • Kinetics
  • Hyperthermia, Induced
  • Humans
  • Glioma
  • Dogs
 

Citation

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Hauck, M. L., Dewhirst, M. W., & Zalutsky, M. R. (1996). The effects of clinically relevant hyperthermic temperatures on the kinetic binding parameters of a monoclonal antibody. Nucl Med Biol, 23(4), 551–557. https://doi.org/10.1016/0969-8051(96)00039-x
Hauck, M. L., M. W. Dewhirst, and M. R. Zalutsky. “The effects of clinically relevant hyperthermic temperatures on the kinetic binding parameters of a monoclonal antibody.Nucl Med Biol 23, no. 4 (May 1996): 551–57. https://doi.org/10.1016/0969-8051(96)00039-x.
Hauck, M. L., et al. “The effects of clinically relevant hyperthermic temperatures on the kinetic binding parameters of a monoclonal antibody.Nucl Med Biol, vol. 23, no. 4, May 1996, pp. 551–57. Pubmed, doi:10.1016/0969-8051(96)00039-x.
Journal cover image

Published In

Nucl Med Biol

DOI

ISSN

0969-8051

Publication Date

May 1996

Volume

23

Issue

4

Start / End Page

551 / 557

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Osteosarcoma
  • Nuclear Medicine & Medical Imaging
  • Mice, Nude
  • Mice
  • Kinetics
  • Hyperthermia, Induced
  • Humans
  • Glioma
  • Dogs