Identification of a high-affinity RNA-binding site for the human immunodeficiency virus type 1 Rev protein.

Published

Journal Article

Expression of the structural proteins of human immunodeficiency virus type 1 requires the direct interaction of multiple copies of the viral Rev protein with its highly structured RNA target sequence, the Rev response element (RRE). Nucleotides critical for Rev monomer binding have been mapped by chemical interference to a single site flanking the base of an RNA helix (stem IIB) located within the 234-nucleotide RRE. Binding of additional Rev molecules to an RRE probe did not require any RNA primary sequence information detectable by modification interference beyond that required for binding of a single Rev protein molecule. A synthetic 29-nucleotide RNA molecule designed to incorporate nucleotides identified as critical for Rev binding retained the ability to bind Rev specifically and, therefore, represents a minimal Rev-binding site. We propose that Rev binding to the RRE initiates with the direct interaction of a Rev monomer with a high-affinity binding site located at the base of the IIB stem of the RRE. The subsequent formation of Rev multimers on the RRE appears, in contrast, primarily driven by specific protein-protein interactions.

Full Text

Duke Authors

Cited Authors

  • Tiley, LS; Malim, MH; Tewary, HK; Stockley, PG; Cullen, BR

Published Date

  • January 15, 1992

Published In

Volume / Issue

  • 89 / 2

Start / End Page

  • 758 - 762

PubMed ID

  • 1731351

Pubmed Central ID

  • 1731351

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.89.2.758

Language

  • eng

Conference Location

  • United States