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Functional organization of the Bel-1 trans activator of human foamy virus.

Publication ,  Journal Article
He, F; Sun, JD; Garrett, ED; Cullen, BR
Published in: Journal of virology
April 1993

Human foamy virus encodes a 300-amino-acid nuclear regulatory protein termed Bel-1 that is required for human foamy virus replication in culture. Bel-1 is a potent trans-activator of gene expression directed by the homologous HFV long terminal repeat as well as the long terminal repeat of human immunodeficiency virus type 1. We have used mutational analysis to define several discrete functional domains within Bel-1. The C-terminal approximately 50 amino acids of Bel-1 are shown to be essential for Bel-1 activity but can be effectively substituted by the C-terminal activation domain of VP16. We therefore conclude that the Bel-1 C terminus forms part of an activation domain. Mutations within a central, approximately 100-amino-acid segment of Bel-1 preclude trans-activation by either Bel-1 or the Bel-1/VP16 chimera. These sequences are therefore proposed to direct the interaction of Bel-1 with its viral DNA target sequences. A short Bel-1 segment located between the activation and binding domains is shown to mediate the nuclear localization of this regulatory protein. Although the functional organization of Bel-1 therefore appears comparable to that reported for other eukaryotic transcriptional activators, Bel-1 does not contain sequences homologous to known transcriptional activation or DNA-binding motifs.

Published In

Journal of virology

DOI

EISSN

1098-5514

ISSN

0022-538X

Publication Date

April 1993

Volume

67

Issue

4

Start / End Page

1896 / 1904

Related Subject Headings

  • Virology
  • Transcriptional Activation
  • Transcription Factors
  • Trans-Activators
  • Spumavirus
  • Sequence Deletion
  • Sequence Alignment
  • Retroviridae Proteins
  • Recombinant Fusion Proteins
  • Promoter Regions, Genetic
 

Citation

APA
Chicago
ICMJE
MLA
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He, F., Sun, J. D., Garrett, E. D., & Cullen, B. R. (1993). Functional organization of the Bel-1 trans activator of human foamy virus. Journal of Virology, 67(4), 1896–1904. https://doi.org/10.1128/jvi.67.4.1896-1904.1993
He, F., J. D. Sun, E. D. Garrett, and B. R. Cullen. “Functional organization of the Bel-1 trans activator of human foamy virus.Journal of Virology 67, no. 4 (April 1993): 1896–1904. https://doi.org/10.1128/jvi.67.4.1896-1904.1993.
He F, Sun JD, Garrett ED, Cullen BR. Functional organization of the Bel-1 trans activator of human foamy virus. Journal of virology. 1993 Apr;67(4):1896–904.
He, F., et al. “Functional organization of the Bel-1 trans activator of human foamy virus.Journal of Virology, vol. 67, no. 4, Apr. 1993, pp. 1896–904. Epmc, doi:10.1128/jvi.67.4.1896-1904.1993.
He F, Sun JD, Garrett ED, Cullen BR. Functional organization of the Bel-1 trans activator of human foamy virus. Journal of virology. 1993 Apr;67(4):1896–1904.

Published In

Journal of virology

DOI

EISSN

1098-5514

ISSN

0022-538X

Publication Date

April 1993

Volume

67

Issue

4

Start / End Page

1896 / 1904

Related Subject Headings

  • Virology
  • Transcriptional Activation
  • Transcription Factors
  • Trans-Activators
  • Spumavirus
  • Sequence Deletion
  • Sequence Alignment
  • Retroviridae Proteins
  • Recombinant Fusion Proteins
  • Promoter Regions, Genetic