Exon junction complexes mediate the enhancing effect of splicing on mRNA expression.

Published

Journal Article

Intron-containing genes are generally expressed more effectively in human cells than are intronless versions of the same gene. We have asked whether this effect is due directly to splicing or instead reflects the action of components of the exon junction complex (EJC) that is assembled at splice junctions after splicing is completed. Here, we show that intron removal does not enhance gene expression if EJC formation is blocked. Conversely, RNA tethering of the EJC components SRm160 or RNPS1 boosts the expression of intronless mRNAs but not of spliced mRNAs. Splicing and RNPS1 tethering are shown to enhance the same steps in mRNA biogenesis and function, including mRNA 3' end processing and translation. Together, these data argue that the EJC is primarily responsible for the positive effect of splicing on gene expression.

Full Text

Duke Authors

Cited Authors

  • Wiegand, HL; Lu, S; Cullen, BR

Published Date

  • September 30, 2003

Published In

Volume / Issue

  • 100 / 20

Start / End Page

  • 11327 - 11332

PubMed ID

  • 12972633

Pubmed Central ID

  • 12972633

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.1934877100

Language

  • eng

Conference Location

  • United States