Thermal denaturation of DNA from bromodeoxyuridine substituted cells.

Published

Journal Article

The thermal denaturation of DNA from cell lines extensively substituted with bromodeoxyuridine has been examined spectrophotometrically over a wide range in ionic strength and by thermal elution from hydroxyapatite columns. BrdU substitution stabliizes DNA at all ionic strengths between 7.5 mM and 1350 mM potassium ion concentration, although a plot of log ionic strength vs Tm deviates from linearity above 150 mM. This nonlinearity is most pronounced with BrdU-substituted DNAs, resulting in a lowered delta Tm between unsubstituted and substituted DNA with increasing ionic strength. DMSO is shown to decrease the Tm of both unsubstituted and BrdU-substituted DNA equally, at a rate of .5 degrees C per 1% DMSO.

Full Text

Duke Authors

Cited Authors

  • Cullen, BR; Bick, MD

Published Date

  • January 1, 1976

Published In

Volume / Issue

  • 3 / 1

Start / End Page

  • 49 - 62

PubMed ID

  • 1250706

Pubmed Central ID

  • 1250706

International Standard Serial Number (ISSN)

  • 0305-1048

Digital Object Identifier (DOI)

  • 10.1093/nar/3.1.49

Language

  • eng

Conference Location

  • England