Thermal denaturation of DNA from bromodeoxyuridine substituted cells.
Journal Article (Journal Article)
The thermal denaturation of DNA from cell lines extensively substituted with bromodeoxyuridine has been examined spectrophotometrically over a wide range in ionic strength and by thermal elution from hydroxyapatite columns. BrdU substitution stabliizes DNA at all ionic strengths between 7.5 mM and 1350 mM potassium ion concentration, although a plot of log ionic strength vs Tm deviates from linearity above 150 mM. This nonlinearity is most pronounced with BrdU-substituted DNAs, resulting in a lowered delta Tm between unsubstituted and substituted DNA with increasing ionic strength. DMSO is shown to decrease the Tm of both unsubstituted and BrdU-substituted DNA equally, at a rate of .5 degrees C per 1% DMSO.
Full Text
Duke Authors
Cited Authors
- Cullen, BR; Bick, MD
Published Date
- January 1, 1976
Published In
Volume / Issue
- 3 / 1
Start / End Page
- 49 - 62
PubMed ID
- 1250706
Pubmed Central ID
- PMC342876
International Standard Serial Number (ISSN)
- 0305-1048
Digital Object Identifier (DOI)
- 10.1093/nar/3.1.49
Language
- eng
Conference Location
- England