Characterization of human interferon-gamma and human interleukin-2 from recombinant mammalian cell lines and peripheral blood lymphocytes.

Published

Journal Article

Human interleukin-2 (IL-2) and human interferon-gamma (IF-gamma) isolated from transfected heterologous cell lines were structurally compared with their natural counterparts isolated from peripheral blood lymphocytes (PBL). Several forms of IL-2 and IFN-gamma that were primarily due to differential glycosylation were produced by all cell types studied. Comparable molecular weights and sugar compositions were found for variants of natural and mammalian-derived recombinant IL-2, and variants of natural and mammalian-derived recombinant IFN-gamma. Furthermore, the specific biological activities of the natural and mammalian-derived recombinant lymphokines were similar and comparable to the specific activity of the Escherichia coli-derived recombinant proteins.

Full Text

Duke Authors

Cited Authors

  • Riske, FJ; Cullen, BR; Chizzonite, R

Published Date

  • June 1991

Published In

Volume / Issue

  • 10 / 3

Start / End Page

  • 213 - 218

PubMed ID

  • 1909189

Pubmed Central ID

  • 1909189

International Standard Serial Number (ISSN)

  • 1056-5477

Language

  • eng

Conference Location

  • United States