Specific binding of the human T-cell leukemia virus type I Rex protein to a short RNA sequence located within the Rex-response element.

Journal Article (Journal Article)

Expression of the structural proteins of human T-cell leukemia virus type I is dependent upon the interaction of the viral Rex trans activator with its highly structured cis-acting RNA target sequence, the 254-nucleotide Rex-response element. Nucleotides critical for Rex binding in vitro have been mapped by modification interference analysis to a discrete 12-nucleotide RNA sequence that is predicted to form a stem-bulge-stem structure. This minimal RNA binding site was sufficient to mediate specific Rex binding in vitro when analyzed in the context of a short RNA probe. The critical importance of this short RNA sequence in mediating Rex function in vivo is supported by its complete conservation among all primate T-cell leukemia virus isolates.

Full Text

Duke Authors

Cited Authors

  • Bogerd, HP; Tiley, LS; Cullen, BR

Published Date

  • December 1992

Published In

Volume / Issue

  • 66 / 12

Start / End Page

  • 7572 - 7575

PubMed ID

  • 1433531

Pubmed Central ID

  • PMC240472

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/JVI.66.12.7572-7575.1992


  • eng

Conference Location

  • United States