APC-mediated downregulation of beta-catenin activity involves nuclear sequestration and nuclear export.
Mutational inactivation of adenomatous polyposis coli (APC) initiates most colon carcinomas. APC functions include targeting cytoplasmic beta-catenin, a Wnt pathway mediator, for proteolysis. Although APC shuttles between cytoplasm and nucleus, the role of nuclear APC protein, particularly with respect to nuclear beta-catenin levels and activity, remains unclear. Here, we demonstrate that APC lacking functional nuclear localization signals (NLSs) or nuclear export signals (NESs) does not effectively downregulate nuclear beta-catenin levels; neither does wild-type APC when nuclear export is blocked. While APC bearing mutated NLSs could not downregulate beta-catenin-mediated transcriptional activation, APC lacking NESs remained active. Consistent with the hypothesis that nuclear APC lacking NESs can inhibit beta-catenin function by sequestration, we show that endogenous APC and beta-catenin proteins interact within the nucleus. These data demonstrate that nuclear APC binding to beta-catenin, and then inducing its nuclear export, plays a critical role in the control of nuclear beta-catenin levels and activity.
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Related Subject Headings
- beta Catenin
- Transfection
- Transcriptional Activation
- Transcription Factors
- Trans-Activators
- Protein Transport
- Protein Binding
- Precipitin Tests
- Mutation
- Models, Genetic
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- beta Catenin
- Transfection
- Transcriptional Activation
- Transcription Factors
- Trans-Activators
- Protein Transport
- Protein Binding
- Precipitin Tests
- Mutation
- Models, Genetic