Amphibian transcription factor IIIA proteins contain a sequence element functionally equivalent to the nuclear export signal of human immunodeficiency virus type 1 Rev.

Journal Article

The human immunodeficiency virus type 1 (HIV-1) Rev protein is required for nuclear export of late HIV-1 mRNAs. This function is dependent on the mutationally defined Rev activation domain, which also forms a potent nuclear export signal. Transcription factor IIIA (TFIIIA) binds to 5S rRNA transcripts and this interaction has been proposed to play a role in the efficient nuclear export of 5S rRNA in amphibian oocytes. Here it is reported that amphibian TFIIIA proteins contain a sequence element with homology to the Rev activation domain that effectively substitutes for this domain in inducing the nuclear export of late HIV-1 mRNAs. It is further demonstrated that this TFIIIA sequence element functions as a protein nuclear export signal in both human cells and frog oocytes. Thus, this shared protein motif may play an analogous role in mediating the nuclear export of both late HIV-1 RNAs and 5S rRNA transcripts.

Full Text

Duke Authors

Cited Authors

  • Fridell, RA; Fischer, U; Lührmann, R; Meyer, BE; Meinkoth, JL; Malim, MH; Cullen, BR

Published Date

  • April 2, 1996

Published In

Volume / Issue

  • 93 / 7

Start / End Page

  • 2936 - 2940

PubMed ID

  • 8610146

International Standard Serial Number (ISSN)

  • 0027-8424

Language

  • eng

Conference Location

  • United States