Regulation of the human c-myc gene: 5' noncoding sequences do not affect translation.

Published

Journal Article

The influence of untranslated 5' sequences on c-myc expression was compared by measuring the translational efficiencies of mRNAs which contain leaders derived from exon 1 or intron 1 of the human c-myc gene. Expression plasmids were constructed and introduced into COS cells, and the levels of c-myc mRNA and protein were examined. Our results show that mRNAs transcribed from constructs containing exon 1 or intron 1, which have different folding potential, are translated with approximately equal efficiencies. This suggests that the translation of c-myc mRNA is not controlled by secondary structure alone. In addition, we observed that transcripts in which exon 1 was deleted are not translated more efficiently, but are present at a higher steady-state level. Thus, this example provides evidence for possible control at the transcriptional level. Finally, since the c-myc product was produced in each of our test systems, the results suggest that this protein does not regulate its own transcription or translation via a specific interaction with c-myc exon 1 alone.

Full Text

Duke Authors

Cited Authors

  • Butnick, NZ; Miyamoto, C; Chizzonite, R; Cullen, BR; Ju, G; Skalka, AM

Published Date

  • November 1985

Published In

Volume / Issue

  • 5 / 11

Start / End Page

  • 3009 - 3016

PubMed ID

  • 3018494

Pubmed Central ID

  • 3018494

Electronic International Standard Serial Number (EISSN)

  • 1098-5549

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.5.11.3009

Language

  • eng