MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms.

Journal Article (Journal Article)

MicroRNAs (miRNAs) are endogenously encoded small noncoding RNAs, derived by processing of short RNA hairpins, that can inhibit the translation of mRNAs bearing partially complementary target sequences. In contrast, small interfering RNAs (siRNAs), which are derived by processing of long double-stranded RNAs and are often of exogenous origin, degrade mRNAs bearing fully complementary sequences. Here, we demonstrate that an endogenously encoded human miRNA is able to cleave an mRNA bearing fully complementary target sites, whereas an exogenously supplied siRNA can inhibit the expression of an mRNA bearing partially complementary sequences without inducing detectable RNA cleavage. These data suggest that miRNAs and siRNAs can use similar mechanisms to repress mRNA expression and that the choice of mechanism may be largely or entirely determined by the degree of complementary of the RNA target.

Full Text

Duke Authors

Cited Authors

  • Zeng, Y; Yi, R; Cullen, BR

Published Date

  • August 19, 2003

Published In

Volume / Issue

  • 100 / 17

Start / End Page

  • 9779 - 9784

PubMed ID

  • 12902540

Pubmed Central ID

  • PMC187842

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.1630797100


  • eng

Conference Location

  • United States