Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells.

Published

Journal Article

Animal cells have recently been shown to express a range of approximately 22 nucleotide noncoding RNAs termed micro RNAs (miRNAs). Here, we show that the human mir-30 miRNA can be excised from irrelevant, endogenously transcribed mRNAs encompassing the predicted 71 nucleotide mir-30 precursor. Expression of the mir-30 miRNA specifically blocked the translation in human cells of an mRNA containing artificial mir-30 target sites. Similarly, designed miRNAs were also excised from transcripts encompassing artificial miRNA precursors and could inhibit the expression of mRNAs containing a complementary target site. These data indicate that novel miRNAs can be readily produced in vivo and can be designed to specifically inactivate the expression of selected target genes in human cells.

Full Text

Duke Authors

Cited Authors

  • Zeng, Y; Wagner, EJ; Cullen, BR

Published Date

  • June 2002

Published In

Volume / Issue

  • 9 / 6

Start / End Page

  • 1327 - 1333

PubMed ID

  • 12086629

Pubmed Central ID

  • 12086629

Electronic International Standard Serial Number (EISSN)

  • 1097-4164

International Standard Serial Number (ISSN)

  • 1097-2765

Digital Object Identifier (DOI)

  • 10.1016/s1097-2765(02)00541-5

Language

  • eng