Using viral species specificity to define a critical protein/RNA interaction surface.

Published

Journal Article

The Tap protein mediates the sequence-specific nuclear export of mRNAs bearing the retroviral constitutive transport element (CTE) and also plays a critical role in the sequence nonspecific export of cellular mRNAs. Previously, we have demonstrated that CTE function displays species specificity, that is, the CTE functions in human but not quail cells. Here, we demonstrate that quail Tap fails to support CTE function because it cannot bind the CTE. However, changing a single residue in quail Tap, glutamine 246, to arginine, the residue found in human Tap, rescues both CTE function and CTE binding. This residue, which is located on the exterior of a recently reported molecular structure of Tap, defines a surface on Tap that is critical for CTE binding. These data emphasize the potential importance of cross-species genetic complementation in the identification and characterization of cellular factors that are critical for different aspects of viral replication.

Full Text

Duke Authors

Cited Authors

  • Coburn, GA; Wiegand, HL; Kang, Y; Ho, DN; Georgiadis, MM; Cullen, BR

Published Date

  • May 15, 2001

Published In

Volume / Issue

  • 15 / 10

Start / End Page

  • 1194 - 1205

PubMed ID

  • 11358864

Pubmed Central ID

  • 11358864

International Standard Serial Number (ISSN)

  • 0890-9369

Digital Object Identifier (DOI)

  • 10.1101/gad.888201

Language

  • eng

Conference Location

  • United States