Mutational analysis of the conserved basic domain of human immunodeficiency virus tat protein.

Journal Article (Journal Article)

The tat trans-activators encoded by the known strains of primate immunodeficiency virus share a conserved, highly basic protein domain. Mutagenesis of this sequence in the tat gene of human immunodeficiency virus type 1 is shown here to reduce, but not eliminate, the trans-activation of human immunodeficiency virus type 1-specific gene expression. The degree of inhibition is shown to vary in a dose-dependent manner and is most marked at low levels of tat expression. Multiple mutations of the basic domain of tat were found to impair both the in vivo stability and the nuclear localization of the tat protein. It is proposed that this protein domain serves to efficiently target the tat gene product to its appropriate site or substrate within the nucleus of expressing cells.

Full Text

Duke Authors

Cited Authors

  • Hauber, J; Malim, MH; Cullen, BR

Published Date

  • March 1989

Published In

Volume / Issue

  • 63 / 3

Start / End Page

  • 1181 - 1187

PubMed ID

  • 2536828

Pubmed Central ID

  • PMC247813

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/JVI.63.3.1181-1187.1989


  • eng

Conference Location

  • United States