Nuclear export of late HIV-1 mRNAs occurs via a cellular protein export pathway.

Published

Journal Article

The Rev protein of HIV-1 is essential for the nuclear export of incompletely spliced viral mRNAs. This action depends on the mutationally defined Rev activation domain, which both binds the nucleoporin-like human cellular cofactor Rab/hRIP and also functions as a nuclear export signal. Protein kinase inhibitor alpha (PKI) also contains a potent nuclear export signal. However, PKI plays no role in nuclear RNA export and instead induces the nuclear export of a specific protein target, the catalytic subunit of cAMP-dependent protein kinase. Here, it is demonstrated that the nuclear export signal of PKI not only binds the Rab/hRIP cofactor specifically but also can effectively substitute for the Rev activation domain in mediating the nuclear export of HIV-1 mRNAs. We conclude that HIV-1 Rev and PKI act through an identical nuclear export pathway and that Rev, rather than using a dedicated RNA export pathway, is instead acting as an adaptor that allows viral mRNAs to access a cellular protein export pathway.

Full Text

Duke Authors

Cited Authors

  • Fridell, RA; Bogerd, HP; Cullen, BR

Published Date

  • April 30, 1996

Published In

Volume / Issue

  • 93 / 9

Start / End Page

  • 4421 - 4424

PubMed ID

  • 8633082

Pubmed Central ID

  • 8633082

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.93.9.4421

Language

  • eng

Conference Location

  • United States