Expression of transfected DNA in avian cells can be enhanced in trans by retroviral infection.

Published

Journal Article

Using a quantitative S1 nuclease protection assay, we demonstrated that acute or chronic infection of avian cells enhances expression of an exogenously introduced rat preproinsulin II gene by approximately equal to 50-fold. The degree of enhancement is shown to vary with the transfection technique used but is independent of the transcription control region of the transfected gene. We conclude that retroviral infection of avian cells enhances expression of transfected DNA in trans by facilitating the uptake of DNA rather than by activating the transfected promoter.

Full Text

Duke Authors

Cited Authors

  • Cullen, BR; Katz, RA; Ju, G

Published Date

  • July 1, 1985

Published In

Volume / Issue

  • 5 / 7

Start / End Page

  • 1804 - 1807

PubMed ID

  • 2991753

Pubmed Central ID

  • 2991753

Electronic International Standard Serial Number (EISSN)

  • 1098-5549

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.5.7.1804

Language

  • eng