Secreted placental alkaline phosphatase: a powerful new quantitative indicator of gene expression in eukaryotic cells.

Published

Journal Article

This paper describes a novel eukaryotic reporter gene, secreted alkaline phosphatase (SEAP). In transient expression experiments using transfected mammalian cells, we demonstrate that SEAP yields results that are qualitatively and quantitatively similar, at both the mRNA and protein levels, to parallel results obtained using established reporter genes. However, SEAP offers significant advantages in terms of ease of assay and assay expense, and also has the potential for quantitative assay at levels as low as 0.2 pg/ml of culture medium. These attributes suggest that SEAP may have general utility in experiments which rely on the accurate measurement of reporter gene expression levels.

Full Text

Duke Authors

Cited Authors

  • Berger, J; Hauber, J; Hauber, R; Geiger, R; Cullen, BR

Published Date

  • June 1988

Published In

Volume / Issue

  • 66 / 1

Start / End Page

  • 1 - 10

PubMed ID

  • 3417148

Pubmed Central ID

  • 3417148

Electronic International Standard Serial Number (EISSN)

  • 1879-0038

International Standard Serial Number (ISSN)

  • 0378-1119

Digital Object Identifier (DOI)

  • 10.1016/0378-1119(88)90219-3

Language

  • eng