Secreted placental alkaline phosphatase: a powerful new quantitative indicator of gene expression in eukaryotic cells.
Journal Article
This paper describes a novel eukaryotic reporter gene, secreted alkaline phosphatase (SEAP). In transient expression experiments using transfected mammalian cells, we demonstrate that SEAP yields results that are qualitatively and quantitatively similar, at both the mRNA and protein levels, to parallel results obtained using established reporter genes. However, SEAP offers significant advantages in terms of ease of assay and assay expense, and also has the potential for quantitative assay at levels as low as 0.2 pg/ml of culture medium. These attributes suggest that SEAP may have general utility in experiments which rely on the accurate measurement of reporter gene expression levels.
Full Text
Duke Authors
Cited Authors
- Berger, J; Hauber, J; Hauber, R; Geiger, R; Cullen, BR
Published Date
- June 15, 1988
Published In
Volume / Issue
- 66 / 1
Start / End Page
- 1 - 10
PubMed ID
- 3417148
Pubmed Central ID
- 3417148
International Standard Serial Number (ISSN)
- 0378-1119
Digital Object Identifier (DOI)
- 10.1016/0378-1119(88)90219-3
Language
- eng
Conference Location
- Netherlands