Transcriptional trans activators of human and simian foamy viruses contain a small, highly conserved activation domain.

Journal Article (Journal Article)

The Bel-1 protein of human foamy virus is a potent transcriptional trans activator of its homologous long terminal repeat promoter element. Here, we demonstrate that Bel-1 can also efficiently activate gene expression when targeted to a heterologous promoter by fusion to the DNA-binding motif of the yeast GAL4 protein. Analysis of a series of deletion mutants of Bel-1 generated in this hybrid protein context suggests the presence of a single transcription activation domain that is fully contained within a discrete, approximately 30-amino-acid segment located proximal to the Bel-1 carboxy terminus. Although this short motif can be shown to function effectively in eukaryotic cells of mammalian, avian, and fungal origin, it does not bear any evident sequence homology to the known classes of eukaryotic activation domain. However, this Bel-1 activation domain was found to be fully conserved, in terms of both biological activity and location, in the distantly related Taf trans activator of simian foamy virus type 1.

Full Text

Duke Authors

Cited Authors

  • Garrett, ED; He, F; Bogerd, HP; Cullen, BR

Published Date

  • November 1993

Published In

Volume / Issue

  • 67 / 11

Start / End Page

  • 6824 - 6827

PubMed ID

  • 8411385

Pubmed Central ID

  • PMC238126

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/JVI.67.11.6824-6827.1993


  • eng

Conference Location

  • United States