Assaying nuclear messenger RNA export in human cells.

Published

Journal Article

This chapter describes a simple method for the analysis of nuclear messenger RNA (mRNA) export in human cells in culture. The assay described relies on the observation that mRNA molecules containing an intron are generally retained in the nucleus until splicing is completed. Upon sequestration of the cat indicator gene in a single intron located 5' to an mRNA cap site, CAT protein expression becomes dependent on the specific recruitment of a nuclear RNA export factor to the unspliced cat RNA via an inserted RNA binding site. This site can be a natural, high affinity RNA target for the nuclear export factor or alternately the export factor can be tethered to the unspliced cat mRNA by fusion to a heterologous RNA binding domain.

Full Text

Duke Authors

Cited Authors

  • Cullen, BR

Published Date

  • 2004

Published In

Volume / Issue

  • 257 /

Start / End Page

  • 85 - 92

PubMed ID

  • 14769998

Pubmed Central ID

  • 14769998

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1385/1-59259-750-5:085

Language

  • eng

Conference Location

  • United States