Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs.

Published

Journal Article

MicroRNAs (miRNAs) are initially expressed as long transcripts that are processed in the nucleus to yield approximately 65-nucleotide (nt) RNA hairpin intermediates, termed pre-miRNAs, that are exported to the cytoplasm for additional processing to yield mature, approximately 22-nt miRNAs. Here, we demonstrate that human pre-miRNA nuclear export, and miRNA function, are dependent on Exportin-5. Exportin-5 can bind pre-miRNAs specifically in vitro, but only in the presence of the Ran-GTP cofactor. Short hairpin RNAs, artificial pre-miRNA analogs used to express small interfering RNAs, also depend on Exportin-5 for nuclear export. Together, these findings define an additional cellular cofactor required for miRNA biogenesis and function.

Full Text

Duke Authors

Cited Authors

  • Yi, R; Qin, Y; Macara, IG; Cullen, BR

Published Date

  • December 15, 2003

Published In

Volume / Issue

  • 17 / 24

Start / End Page

  • 3011 - 3016

PubMed ID

  • 14681208

Pubmed Central ID

  • 14681208

International Standard Serial Number (ISSN)

  • 0890-9369

Digital Object Identifier (DOI)

  • 10.1101/gad.1158803

Language

  • eng

Conference Location

  • United States