Potent and specific inhibition of human immunodeficiency virus type 1 replication by RNA interference.

Published

Journal Article

Synthetic small interfering RNAs (siRNAs) have been shown to induce the degradation of specific mRNA targets in human cells by inducing RNA interference (RNAi). Here, we demonstrate that siRNA duplexes targeted against the essential Tat and Rev regulatory proteins encoded by human immunodeficiency virus type 1 (HIV-1) can specifically block Tat and Rev expression and function. More importantly, we show that these same siRNAs can effectively inhibit HIV-1 gene expression and replication in cell cultures, including those of human T-cell lines and primary lymphocytes. These observations demonstrate that RNAi can effectively block virus replication in human cells and raise the possibility that RNAi could provide an important innate protective response, particularly against viruses that express double-stranded RNAs as part of their replication cycle.

Full Text

Duke Authors

Cited Authors

  • Coburn, GA; Cullen, BR

Published Date

  • September 2002

Published In

Volume / Issue

  • 76 / 18

Start / End Page

  • 9225 - 9231

PubMed ID

  • 12186906

Pubmed Central ID

  • 12186906

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/jvi.76.18.9225-9231.2002

Language

  • eng

Conference Location

  • United States