Identification of a novel human zinc finger protein that specifically interacts with the activation domain of lentiviral Tat proteins.

Published

Journal Article

Transcriptional activation of HIV-1 gene expression by the viral Tat protein requires the interaction of a cellular cofactor with the Tat activation domain. This domain has been shown to consist of the cysteine-rich and core motifs of HIV-1 Tat and is functionally conserved in the distantly related Tat proteins of HIV-2 and EIAV. Using the yeast two-hybrid system, we have identified a novel human gene product, termed HT2A, that specifically and precisely binds to the activation domain of HIV-1 Tat and that can also interact with the HIV-2 and EIAV Tat proteins in vivo. We present data further demonstrating that the interaction between the activation domain of HIV-1 Tat and the HT2A protein can be readily detected in the mammalian cell nucleus. Sequence analysis demonstrates that HT2A is a novel member of the C3HC4 or ring finger family of zinc finger proteins that includes several known oncogenes and transcription factors. Overall, these data suggest that HT2A may play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo.

Full Text

Duke Authors

Cited Authors

  • Fridell, RA; Harding, LS; Bogerd, HP; Cullen, BR

Published Date

  • June 1995

Published In

Volume / Issue

  • 209 / 2

Start / End Page

  • 347 - 357

PubMed ID

  • 7778269

Pubmed Central ID

  • 7778269

Electronic International Standard Serial Number (EISSN)

  • 2514-4138

International Standard Serial Number (ISSN)

  • 1096-0341

Digital Object Identifier (DOI)

  • 10.1006/viro.1995.1266

Language

  • eng