Genetic analysis indicates that the human foamy virus Bel-1 protein contains a transcription activation domain of the acidic class.

Journal Article (Journal Article)

Human foamy virus encodes a nuclear regulatory protein, termed Bel-1, that serves as a potent activator of viral transcription. Mutational analysis has identified a small, discrete activation domain within Bel-1 that is highly active in both higher and lower eukaryotic cells. Here, we demonstrate that the activation domain of Bel-1 is highly dependent on the ADA2 transcriptional adaptor for biological activity in yeast cells, a property previously shown to be a hallmark of the VP16 class of acidic transcriptional activators (S. L. Berger, B. Pina, N. Silverman, G. A. Marcus, J. Agapite, J. L. Regier, S. J. Triezenberg, and L. Guarente, Cell 70:251-265, 1992). Using genetic selection in yeast cells, we have derived a set of point mutants within the Bel-1 activation domain that display a qualitatively similar loss in activation potential when examined in either yeast or human cells. These data indicate that the Bel-1 activation domain functions similarly in both lower and higher eukaryotes and strongly suggest that Bel-1 belongs to the VP16 class of acidic transcription factors.

Full Text

Duke Authors

Cited Authors

  • Blair, WS; Bogerd, H; Cullen, BR

Published Date

  • June 1994

Published In

Volume / Issue

  • 68 / 6

Start / End Page

  • 3803 - 3808

PubMed ID

  • 8189518

Pubmed Central ID

  • PMC236885

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/JVI.68.6.3803-3808.1994


  • eng

Conference Location

  • United States