Identification of a U5-specific sequence required for efficient polyadenylation within the human immunodeficiency virus long terminal repeat.

Published

Journal Article

Retrovirus mRNAs are normally polyadenylated within the proviral 3' long terminal repeat (LTR). The site of retrovirus transcript polyadenylation is flanked 3' by an LTR-specific sequence termed the U5 region, but the role of U5 in the determination of polyadenylation efficiency has not been addressed. We have used site-directed mutagenesis of a human immunodeficiency virus LTR to map U5 sequences which are required for efficient polyadenylation within the LTR. These LTR U5 region sequences display homology to a motif termed the G-T cluster, which is known to facilitate the efficient polyadenylation of mRNAs encoded by several cellular and viral genes. These results suggest that the LTR U5 region functions in vivo to permit efficient polyadenylation within the proviral 3' LTR.

Full Text

Duke Authors

Cited Authors

  • Böhnlein, S; Hauber, J; Cullen, BR

Published Date

  • January 1989

Published In

Volume / Issue

  • 63 / 1

Start / End Page

  • 421 - 424

PubMed ID

  • 2908926

Pubmed Central ID

  • 2908926

International Standard Serial Number (ISSN)

  • 0022-538X

Language

  • eng

Conference Location

  • United States