A single stem-loop structure within the HTLV-1 Rex response element is sufficient to mediate Rex activity in vivo.

Published

Journal Article

The human T-cell leukemia virus (HTLV-1) Rex protein is required for the cytoplasmic expression of the incompletely spliced transcripts that encode the viral structural proteins. This effect is mediated by a highly structured cis-acting RNA element of 254 nucleotides termed the Rex response element, or RexRE. Here we demonstrate that one of the four known RexRE stem-loop structures as well as a 43-nt segment derived from this element is sufficient to mediate Rex function in vivo. Upon duplication, this stem-loop is shown to function as efficiently as the full-length RexRE. In vitro RNA binding analyses with wildtype and mutagenized RNA show that this stem-loop contains a high affinity binding site for Rex that coincides with a predicted bulge structure in the central part of this stem-loop. These results indicate that a small region of the RexRE containing a high affinity binding site is sufficient to mediate Rex function and suggest that sequences outside of this binding site have no unique role in mediating Rex regulation.

Full Text

Duke Authors

Cited Authors

  • Gröne, M; Hoffmann, E; Berchtold, S; Cullen, BR; Grassmann, R

Published Date

  • October 1994

Published In

Volume / Issue

  • 204 / 1

Start / End Page

  • 144 - 152

PubMed ID

  • 8091649

Pubmed Central ID

  • 8091649

Electronic International Standard Serial Number (EISSN)

  • 1096-0341

International Standard Serial Number (ISSN)

  • 0042-6822

Digital Object Identifier (DOI)

  • 10.1006/viro.1994.1518

Language

  • eng