Effects of chimeric mutants of human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type I Rex on nucleolar targeting signals.

Journal Article

Two chimeric mutant genes derived from rev of human immunodeficiency virus type 1 and rex of human T-cell leukemia virus type I were constructed to investigate the functions of the nucleolar-targeting signals (NOS) in Rev and Rex proteins. A chimeric Rex protein whose NOS region was substituted with the NOS of Rev was located predominantly in the cell nucleolus and functioned like the wild-type protein in the Rex assay system. However, a chimeric Rev with the NOS of Rex abolished Rev function despite its nucleolar localization. This nonfunctional nucleolar-targeting chimeric protein inhibited the function of both Rex and Rev. In the same experimental conditions, this mutant interfered with the localization of the functional Rex in the nucleolus.

Full Text

Duke Authors

Cited Authors

  • Kubota, S; Nosaka, T; Cullen, BR; Maki, M; Hatanaka, M

Published Date

  • May 1991

Published In

Volume / Issue

  • 65 / 5

Start / End Page

  • 2452 - 2456

PubMed ID

  • 2016767

International Standard Serial Number (ISSN)

  • 0022-538X

Language

  • eng

Conference Location

  • United States