HIV-1 structural gene expression requires binding of the Rev trans-activator to its RNA target sequence.

Journal Article

Expression of human immunodeficiency virus type 1 structural proteins requires both the viral Rev trans-activator and its cis-acting RNA target sequence, the Rev response element (RRE). The RRE has been mapped to a conserved region of the HIV-1 env gene and is predicted to form a complex, highly stable RNA stem-loop structure. Site-directed mutagenesis was used to define a small subdomain of the RRE, termed stem-loop II, that is essential for biological activity. Gel retardation assays demonstrated that the Rev trans-activator is a sequence-specific RNA binding protein. The RRE stem-loop II subdomain was found to be both necessary and sufficient for the binding of Rev by the RRE. We propose that the HIV-1 Rev trans-activator belongs to a new class of sequence-specific RNA binding proteins characterized by the presence of an arginine-rich binding motif.

Full Text

Duke Authors

Cited Authors

  • Malim, MH; Tiley, LS; McCarn, DF; Rusche, JR; Hauber, J; Cullen, BR

Published Date

  • February 23, 1990

Published In

Volume / Issue

  • 60 / 4

Start / End Page

  • 675 - 683

PubMed ID

  • 2406030

International Standard Serial Number (ISSN)

  • 0092-8674

Language

  • eng

Conference Location

  • United States