Functional characterization of a complex protein-DNA-binding domain located within the human immunodeficiency virus type 1 long terminal repeat leader region.

Journal Article

Transcriptional trans activation of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) by the viral tat trans activator is mediated by an LTR-specific sequence located immediately 3' to the start of transcription initiation. We have used a range of molecular techniques to examine DNA-protein interactions that occur in the vicinity of this cis-acting sequence. Our results demonstrate the existence of a sequence-specific DNA-protein interaction involving the HIV-1 leader DNA and map this binding event to between -2 and +21 base pairs relative to the HIV-1 LTR transcription start site. Evidence suggesting that this interaction involves three distinct protein-DNA contact sites extending along one side of the DNA helix is presented. Mutation of these sites was found to ablate protein-DNA binding yet was observed to have no effect on either the basal or tat trans-activated level of HIV-1 LTR-specific gene expression. We therefore conclude that this DNA-protein interaction has a function distinct from the regulation of HIV-1 LTR-specific gene expression.

Full Text

Duke Authors

Cited Authors

  • Malim, MH; Fenrick, R; Ballard, DW; Hauber, J; Böhnlein, E; Cullen, BR

Published Date

  • August 1, 1989

Published In

Volume / Issue

  • 63 / 8

Start / End Page

  • 3213 - 3219

PubMed ID

  • 2545899

International Standard Serial Number (ISSN)

  • 0022-538X

Language

  • eng

Conference Location

  • United States