A rapid and simple procedure for the preparation of homogeneously labeled DNA probes.
Journal Article (Journal Article)
This report describes a novel technique for the preparation of homogeneously labeled DNA probes. The procedure uses a heteroduplex intermediate, which is directly prepared from a double-stranded plasmid, and therefore requires neither sub-cloning of the fragment of interest into a specialized vector nor the use of a synthetic primer. The technical advantages of the procedure are demonstrated by preparation of a probe able to determine the precise location and efficiency of utilization of the polyadenylation site used during transcription of the rat preproinsulin II gene in a transfected avian cell line.
Full Text
Duke Authors
Cited Authors
- Cullen, BR
Published Date
- 1986
Published In
Volume / Issue
- 43 / 3
Start / End Page
- 305 - 310
PubMed ID
- 3017814
International Standard Serial Number (ISSN)
- 0378-1119
Digital Object Identifier (DOI)
- 10.1016/0378-1119(86)90221-0
Language
- eng
Conference Location
- Netherlands