A rapid and simple procedure for the preparation of homogeneously labeled DNA probes.

Journal Article

This report describes a novel technique for the preparation of homogeneously labeled DNA probes. The procedure uses a heteroduplex intermediate, which is directly prepared from a double-stranded plasmid, and therefore requires neither sub-cloning of the fragment of interest into a specialized vector nor the use of a synthetic primer. The technical advantages of the procedure are demonstrated by preparation of a probe able to determine the precise location and efficiency of utilization of the polyadenylation site used during transcription of the rat preproinsulin II gene in a transfected avian cell line.

Full Text

Duke Authors

Cited Authors

  • Cullen, BR

Published Date

  • 1986

Published In

Volume / Issue

  • 43 / 3

Start / End Page

  • 305 - 310

PubMed ID

  • 3017814

International Standard Serial Number (ISSN)

  • 0378-1119

Language

  • eng

Conference Location

  • Netherlands