Protein patterns of early mouse embryos during development.

Published

Journal Article

Using high resolution two-dimensional polyacrylamide gel electrophoresis, the major protein species (labeled in vitro with 35S-methionine) of mouse embryos were examined starting with the unfertilized egg up to the blastocyst stage (fourth day of development). The analysis was then continued using in vitro culture techniques up to the 10th equivalent gestation day. At all periods of development distinct protein changes could be seen. However, major alterations in the protein synthesis pattern were noted between the 2nd and 3rd day in vivo and around the 8th equivalent gestation day in vitro. A complete series of gels is presented such that proteins can be easily identified in terms of their molecular weights and isoelectric points.

Full Text

Duke Authors

Cited Authors

  • Cullen, BR; Emigholz, K; Monahan, JJ

Published Date

  • 1980

Published In

Volume / Issue

  • 17 / 3

Start / End Page

  • 151 - 160

PubMed ID

  • 7450326

Pubmed Central ID

  • 7450326

International Standard Serial Number (ISSN)

  • 0301-4681

Language

  • eng

Conference Location

  • England