Effect of intron size on splicing efficiency in retroviral transcripts.

Published

Journal Article

To study the effect of intron size on splicing efficiency we have varied the size of the avian leukosis virus (ALV) env mRNA intron in a cloned ALV genome. This was accomplished by deletion of ALV sequences or insertion of phage lambda DNA. The effect of these modifications on splicing was analyzed by microinjection of the modified clones into RSV(-) chicken cells. Viral env mRNA when transcribed and properly spliced within these cells complemented the RSV(-) env deficiency leading to the production of focus forming units. Using this assay it was shown that deletion of up to 3.7 kb of the 4.68 kb env intron did not inhibit correct splicing nor did insertion of up to 8 kb of phage lambda DNA prevent splicing. Our results indicate that intron size can be varied over a wide range without preventing splicing.

Full Text

Duke Authors

Cited Authors

  • Cullen, BR; Kopchick, JJ; Stacey, DW

Published Date

  • October 1, 1982

Published In

Volume / Issue

  • 10 / 19

Start / End Page

  • 6177 - 6190

PubMed ID

  • 6292870

Pubmed Central ID

  • 6292870

Electronic International Standard Serial Number (EISSN)

  • 1362-4962

International Standard Serial Number (ISSN)

  • 0305-1048

Digital Object Identifier (DOI)

  • 10.1093/nar/10.19.6177

Language

  • eng