Structural and functional analysis of the visna virus Rev-response element.

Published

Journal Article

The distantly related lentiviruses human immunodeficiency virus type 1 (HIV-1) and visna virus each encode a posttranscriptional regulatory protein, termed Rev, that is critical for expression of the viral structural proteins. We genetically mapped the cis-acting target sequence for visna virus Rev, the visna virus Rev-response element or RRE-V, to a complex 176-nucleotide RNA stem-loop structure that coincides with sequences encoding the N terminus of the transmembrane component of envelope. The computer-predicted structure of the RRE-V was validated by in vitro analysis of structure-specific RNase cleavage patterns. The visna virus Rev protein was shown to interact specifically with the genetically defined RRE-V in vitro but was unable to bind the HIV-1 RRE. Similarly, HIV-1 Rev was also unable to bind the RRE-V specifically. We therefore conclude that the HIV-1 and visna virus Rev proteins, while functionally analogous, nevertheless display distinct RNA sequence specificities. These findings provide a biochemical explanation for the observation that these two viral regulatory proteins are functional only in the homologous viral system.

Full Text

Duke Authors

Cited Authors

  • Tiley, LS; Cullen, BR

Published Date

  • June 1992

Published In

Volume / Issue

  • 66 / 6

Start / End Page

  • 3609 - 3615

PubMed ID

  • 1316470

Pubmed Central ID

  • 1316470

International Standard Serial Number (ISSN)

  • 0022-538X

Language

  • eng

Conference Location

  • United States