Functional analysis of interactions between Tat and the trans-activation response element of human immunodeficiency virus type 1 in cells.

Journal Article

Transcriptional trans-activation of the human immunodeficiency virus type 1 long terminal repeat requires that the virally encoded Tat effector interacts with its target trans-activation response element (TAR) RNA stem-loop. Although the arginine-rich region of Tat from amino acids 49 to 59 is sufficient to bind to TAR RNA in vitro, the RNA-binding domain of Tat has not been defined in vivo. Human immunodeficiency virus type 1 also encodes the Rev protein, which acts through an RNA stem-loop called the Rev-response element to transport unspliced and singly spliced viral RNA species from the nucleus to the cytoplasm. To map the RNA-binding domain of Tat, we performed assays that relied on Rev function using the heterologous RNA-tethering mechanism of Tat and the TAR. By examining the effects of selected targeted mutations of Tat on the abilities of hybrid Tat/Rev proteins to rescue the expression of unspliced mRNA via the TAR, we demonstrated that residues throughout the N-terminal 59 amino acids of Tat are required for binding of Tat and TAR RNA in vivo.

Full Text

Duke Authors

Cited Authors

  • Luo, Y; Madore, SJ; Parslow, TG; Cullen, BR; Peterlin, BM

Published Date

  • September 1993

Published In

Volume / Issue

  • 67 / 9

Start / End Page

  • 5617 - 5622

PubMed ID

  • 8350414

International Standard Serial Number (ISSN)

  • 0022-538X

Language

  • eng

Conference Location

  • United States