Expression of active, membrane-bound human placental alkaline phosphatase by transfected simian cells.

Journal Article

Human placental alkaline phosphatase (PALPase) has been transiently expressed in simian (COS) cells by transfection with a eukaryotic expression vector containing the corresponding cDNA. The level of expression of PALPase was high, and it was produced in an enzymatically active form. The bulk of PALPase was associated with the cell membrane as shown by immunocytochemistry and subcellular fractionation studies. The PALPase produced by transfected COS cells, like PALPase in human tissue, was specifically released from the intact cells in a hydrophilic form by phosphatidylinositol-specific phospholipase C and is, therefore, apparently attached to the outer membrane by means of a phosphatidylinositol-glycan. Transfected COS cells appear to be an excellent model for elucidating the mechanism of attachment of this phosphatidylinositol-glycan to a protein moiety.

Full Text

Duke Authors

Cited Authors

  • Berger, J; Howard, AD; Gerber, L; Cullen, BR; Udenfriend, S

Published Date

  • July 1, 1987

Published In

Volume / Issue

  • 84 / 14

Start / End Page

  • 4885 - 4889

PubMed ID

  • 3474633

International Standard Serial Number (ISSN)

  • 0027-8424

Language

  • eng

Conference Location

  • United States